Metabolic Profiling and Comparative Proteomic Insight in Respect of Amidases during Iprodione Biodegradation

The fungicide iprodione (IPR) (3-(3,5-dichlorophenyl) N-isopropyl-2,4-dioxoimidazolidine-1-carboxamide) is a highly toxic compound. Although IPR has been restricted, it still being applied in many places around the world, constituting an environmental risk. biodegradation of attractive option for reducing its residues. In this study, we isolated thirteen IPR-tolerant bacteria from biopurification system designed to treat pesticides. A study using different strains was comparatively evaluated, and best degradation rate presented by Achromobacter sp. C1 with half-life (T1/2) 9 days. Based on nano-LC-MS/MS analysis strains, proteins solely expressed treatment were identified highlighting strain C1, 445 primarily involved biosynthesis secondary metabolites microbial metabolism diverse environments. Differentially protein amidases six metabolic pathways. Interestingly, formamidase inhibited while other cyclases, i.e., amidase mandelamide hydrolase, overexpressed, thereby minimizing effect C1. dynamic changes profiles that degrade have poorly studied; therefore, our results offer new insight into IPR-degrading microorganisms, special attention paid amidases.